Figure 3
From: Dysregulated Wnt and NFAT signaling in a Parkinson’s disease LRRK2 G2019S knock-in model
NFATc1 signaling changes in wild type, LRRK2 KO and LRRK2 G2019S KI mice in vivo over time. Mice were injected intracranially at P0 with a lentiviral biosensor for NFATc1 to detect NFATc1 signaling activities. Repeated measures of the resulting bioluminescence signal were detected over a period of 28 weeks in wild type (WT), LRRK2 knockout (KO) and LRRK2 G2019S knock-in (G2019S) mice. The data is shown as mean relative luciferase activity ± SEM, normalized to WT set to one (A, B, and C). Sex separation reveals differences between LRRK2 genotypes for only male (B) and only female mice (C). The effect of time is shown as mean relative luciferase activity ± SEM, normalized to week one set to one for each individual LRRK2 genotype (D, E, and F). Sex related differences are represented as mean raw luciferase signal ± SEM in photons per seconds (G). Statistical significance, indicated as ****P < 0.0001, ***P < 0.0005, and **P < 0.005 was tested for genotype differences and effect of time by mixed-effects analysis, and the impact of sex on basal in vivo NFATc1 signaling was analyzed by unpaired t-test with Welch’s correction.
