Figure 4
From: Dysregulated Wnt and NFAT signaling in a Parkinson’s disease LRRK2 G2019S knock-in model
GFP staining of injected and non-injected mice with the TCF/LEF biosensor. Mice were injected intracranially at P0 with a lentiviral construct, containing a RE site for TCF/LEF and a second gene for GFP. Six months later, brains were collected from injected (1 and 2) and non-injected control mice (3 and 4). Brains were fixed in PFA, coronally cryosectioned into 40µm thick slices, which were stained via immunohistochemistry for GFP. Positive cells for GFP expression are visible in dark brown indicated by the green arrows. Positive cells were detectable in olfactory bulb (A), cortex (B), hippocampus (C), superior colliculus (D), striatum (E) and thalamus (F and G). No positive cells were measurable in corresponding regions of non-injected control brains. Images in 2 and 4 represent the zoom of indicated squares in 1 and 3. Scale bar for all images is 50µm.
