Figure 7
From: Dysregulated Wnt and NFAT signaling in a Parkinson’s disease LRRK2 G2019S knock-in model
Signaling pathway component changes in the cortex of LRRK2 KO and LRRK2 G2019S KI mice compared to WT mice. Wnt and NFAT signaling component changes in in cortical samples between LRRK2 KO and WT (A1, B1), and LRRK2 G2019S KI and WT (A2, B2) are shown on a transcriptional (A) and protein level (B). Relative expression of relevant gene candidates was detected via quantitative real time PCR. Data is shown as log2fold-change to WT expression. Gapdh and Hprt served as housekeeping genes (A1, A2). Statistical significance shown as *P < 0.05 was tested via unpaired t-test with Welch’s correction with n = 6, 3 males and 3 females (B1, B2). Representative plots are displayed, and data is shown as log2fold-change to WT protein levels. β-actin served as loading control. Statistical significance indicated as ***P < 0.0005, **P < 0.005, and *P < 0.05 was tested via unpaired t-test with Welch’s correction with n = 6, 3 males and 3 females. Western blots in this figure were cut to display all results in a concise format. The original uncut Western Blot images corresponding to this figure are shown in the Supplementary Material 3.
