Figure 9

Signaling pathway component changes in the striatum of LRRK2 KO and LRRK2 G2019S KI mice compared to WT mice . Wnt and NFAT signaling component changes in striatal samples between LRRK2 KO and WT (A1, B1), and LRRK2 G2019S KI and WT (A2, B2) are shown on a transcriptional (A) and protein level (B). Relative expression of relevant gene candidates was detected via quantitative real time PCR. Data is shown as log₂fold-change to WT expression. Gapdh and Hprt served as housekeeping genes (A₁, A₂). Statistical significance shown as **P < 0.005 and *P < 0.05 was tested via unpaired t-test with Welch’s correction with n = 6, 3 males and 3 females (B₁, B₂). Representative plots are displayed, and data is shown as log₂fold-change to WT protein levels. β-actin served as loading control. Statistical significance indicated as ***P < 0.0005, **P < 0.005, and *P < 0.05 was tested via unpaired t-test with Welch’s correction with n = 6, 3 males and 3 females. Western blots in this figure were cut to display all results in a concise format. The original uncut Western Blot images corresponding to this figure are shown in the Supplementary Material 5.