Table 1 Primer information for quantitative real time polymerase chain reaction.
From: Dysregulated Wnt and NFAT signaling in a Parkinson’s disease LRRK2 G2019S knock-in model
Target Gene | Accession Number | Primer Sequence (5’–3’) | Amplicon |
|---|---|---|---|
Wnt5a | NM_009524.4 | (F) GTGCCATGTCTTCCAAGTTC (R) TGCCTGTCTTCGCACCTT | cDNA: 246 bp |
Wnt7a | NM_009527.3 | (F) CGCCAAGGTCTTCGTG (R) AGCCTAAGCTCTCGGAACTGT | cDNA: 191 bp |
β-catenin | NM_007614.3 | (F) CATTGGTGCCCAGGGAGA (R) GATCAGGCAGCCCATCAACT | cDNA: 170 bp |
Axin2 | NM_015732.4 | (F) GCCGACCTCAAGTGCAAACT (R) GCCGGAACCTACGTGATAA | cDNA: 156 bp |
Gsk-3 | NM_019827.7 | (F) TTGGACAAAGGTCTTCCGGC (R) AAGAGTGCAGGTGTGTCTCG | cDNA: 349 bp |
Tcf1 | NM_001313981.1 | (F) ATCTGCTCATGCCCTACCCA (R) GCGGCCTGTGAACTCCT | cDNA: 192 bp |
Nfatc1 | NM_016791.4 | (F) CGGGAAGAAGATGGTGCT (R) CTGGTTGCGGAAAGGTGGTA | cDNA: 169 bp |
Bdnf | NM_001048139.1 | (F) CGGGACGGTCACAGTC (R) CCGAACATACGATTGGGTAG | cDNA: 166 bp |
Gapdh | NM_008084.3 | (F) GCCCAGAACATCATCCCT (R) GTCCTCAGTGTAGCCCAAGA | cDNA: 231 bp gDNA: 315 bp |
Hprt | NM_013556.2 | (F) AGTCCCAGCGTCGTGATTAG (R) GGGCCACAATGTGATG | cDNA: 181 bp |
β-actin (51) | NM_007393.5 | (F) GCCAACCGTGAAAAGATGAC (R) GGCGTGAGGGAGAGCATAG | cDNA: 183 bp gDNA: 637 bp |
Titin | NM_011652.3 | (F) TGCTGGAACTCCCGAGATTG (R) CTCCGCTGTCGCTGCTATCA | gDNA: 137 bp |
WPRE | JC285214 | (F) TTGCTTCCCGTATGGCTTTC (R) GCCGTGGCAATAGGGAGG | vDNA: 202 bp |
