Figure 7

Maintenance of the immune microenvironment in BC-PDEs. Whole slide mIF-stained sections of BC-PDEs were digitised using a Vectra Polaris and analysed in InForm. (A) Representative image of a single PDE stained for CD4, CD8, FOXP3, CK and DAPI. (B) Individual cells were phenotyped into T-Helper (CD4 + ve), T-effector (CD8 + ve), T-regulatory (CD4 + ve FOXP3 + ve), Tumour cells (CK + ve) negative (DAPI + ve). (C) There was no significant difference in cell density/mm2 for T-regulatory, T-effector and T-helper cells in both tumour and stroma in uncultured (48 BC-PDEs) compared to vehicle control (52 BC-PDEs) treated BC-PDEs using Mann–Whitney test, where p < 0.05 (indicated by *) was considered significant. (D) Example image of functional inter-cell distance calculation showing Distance from T-effector to CK cells. Scale bars represent 200 µm.