Figure 4 | Scientific Reports

Figure 4

From: PRSS3/mesotrypsin as a putative regulator of the biophysical characteristics of epidermal keratinocytes in superficial layers

Figure 4

Mesotrypsin induces a flattened morphology and growth arrest in HaCaT cells. (A) Phenotypic depiction of HaCaT cells expressing Venus-mesotrypsin (right) and those without expression (left). Scale bars, 200 µm. (B) Left, Quantitative assessment of cell size. The expression of Venus-mesotrypsin, as opposed to Venus alone, results in a significant increase in cell size. Middle and right, Determination of cell thickness using z-stack images. n = 3, **p < 0.01. ***p < 0.001. Randomly selected 20 cells were analyzed in each experiment. In contrast to Cos7 cells, HaCaT cells exhibit a flattened morphology upon Venus-mesotrypsin expression. (C) The Alamar Blue assay revealed growth arrest in HaCaT cells expressing Venus-mesotrypsin. n = 5, ***p < 0.001.

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