Table 1 Chemical composition of tannin extracts in different fractions (details see below) after Sephadex LH-20 purification. F1: fraction eluted with 30% acetone; F2: fraction eluted with 50% acetone; F3: fraction eluted with 80% acetone; TP: Total polyphenols; TT: Total tannins; TF: Total Flavonoids, HTs: Hydrolysable tannins; PA: Proanthocyanidins; mDP: mean degree of polymerization; PD%: prodelphinidin percentage.

From: Investigating the efficacy of purified tannin extracts from underutilized temperate forages in reducing enteric methane emissions in vitro

Species

Fraction

TP (mg/g)

TT (mg/g)

TF (mg/g)

HTs (mg/g)

PA (mg/g)

mDP

PD %

Birdsfoot trefoil

F1

117.8

100.6

17.2

12.1

88.6

7.8

48.3

Birdsfoot trefoil

F2

239.8

229.1

10.7

39.6

189.6

14.7

51.3

Birdsfoot trefoil

F3

220.0

208.5

11.5

46.3

162.3

13.9

49.4

Sulla

F1

340.2

335.0

5.2

1.1

333.9

12.9

91.1

Sulla

F2

370.8

367.4

3.4

0.8

366.6

17.7

92.3

Sulla

F3

341.6

341.4

0.2

0.6

340.9

24.7

92.6

Big trefoil

F1

264.2

258.1

6.1

0.5

257.5

15.3

86.5

Big trefoil

F2

339.4

331.6

7.8

0.5

331.1

14.7

83.2

Big trefoil

F3

293.3

292.2

1.1

0.9

291.3

21.5

88.0

Salad burnet

F1

172.7

165.5

7.2

151.6

13.9

3.9

41.1

Salad burnet

F2

183.6

182.1

1.5

166.9

15.2

6.7

59.1

Salad burnet

F3

179.1

178.4

0.7

171.1

7.3

4.3

36.9