Table 3 Effect of rutin addition on different tannin extracts on methane production (ml/200 mg DM), gas production (ml/200 mg DM), and methane percentage in total gas (%). Values within parentheses represent the standard error of the mean (SEM).
Extract | Concentration (g/kg DM) | Gas production (ml/200 mg DM) | Methane production (ml/200 mg DM) | Methane percentage in total gas (%) | In vitro organic matter digestibility (%) | Metabolisable energy (MJ/kg DM) | Net energy of lactation (MJ/kg DM) |
|---|---|---|---|---|---|---|---|
Rutin | 10 | 63.9 (0.8) b | 12.6 (0.1) b | 20 (0.5) b | 78 (1.4) b | 10.7 (0.2) b | 6.6 (0.2) b |
Rutin | 20 | 64.2 (1.2) b | 12.8 (0.2) ab | 20.8 (0.4) ab | 76.7 (1.7) ab | 10.4 (0.3) ab | 6.5 (0.2) ab |
Rutin | 30 | 63.4 (1.6) b | 13 (0.2) b | 19.9 (0.4) b | 79.7 (1.4) b | 10.9 (0.2) b | 6.8 (0.2) b |
Birdsfoot Trefoil | 10 | 61.9 (1.9) ab | 12.4 (0.2) ab | 20.2 (0.4) ab | 76.1 (1.9) ab | 10.3 (0.3) ab | 6.4 (0.2) ab |
Birdsfoot Trefoil + Rutin | 10 + 10 | 65.3 (1.6) b | 12.8 (0.4) b | 19.7 (0.5) b | 79.2 (1.3) b | 10.9 (0.2) b | 6.8 (0.2) b |
Birdsfoot Trefoil | 20 | 61.2 (2.1) ab | 13.1 (0.2) b | 20.8 (0.5) b | 77.9 (1.3) b | 10.6 (0.2) b | 6.6 (0.2) b |
Sulla | 10 | 64.8 (1.5) b | 12.6 (0.1) ab | 21.2 (0.4) ab | 74.3 (1) ab | 10 (0.2) ab | 6.2 (0.1) ab |
Sulla + Rutin | 10 + 10 | 63.3 (1.4) b | 12.3 (0.2) ab | 20.3 (0.2) ab | 75.6 (1.1) ab | 10.3 (0.2) ab | 6.3 (0.1) ab |
Sulla | 20 | 59.2 (1.2) ab | 12.2 (0.4) ab | 20.9 (0.6) ab | 73.6 (1.7) ab | 9.9 (0.3) ab | 6.1 (0.2) ab |
Big trefoil | 10 | 60.7 (1.3) ab | 11.6 (0.2) a | 21.1 (0.6) a | 70.7 (1.9) a | 9.5 (0.3) a | 5.8 (0.2) a |
Big trefoil + Rutin | 10 + 10 | 58.5 (1.9) ab | 12.9 (0.3) b | 20 (0.4) b | 79 (1.1) b | 10.8 (0.2) b | 6.7 (0.1) b |
Big trefoil | 20 | 55.1 (2.1) a | 13.3 (0.1) b | 20.8 (0.6) b | 78.6 (1.8) b | 10.7 (0.3) b | 6.7 (0.2) b |
Salad Burnet | 10 | 64.5 (1.3) b | 12.1 (0.5) ab | 19.7 (0.5) ab | 76.2 (1.3) ab | 10.4 (0.2) ab | 6.4 (0.1) ab |
Salad Burnet + Rutin | 10 + 10 | 64 (2.1) b | 13.3 (0.1) b | 20.8 (0.6) b | 78 (1.8) b | 12 (0.2) ab | 7.1 (0.2) b |
Salad Burnet | 20 | 61.4 (1.4) ab | 12.1 (0.5) ab | 19.7 (0.5) ab | 75.6 (1.3) ab | 12.4 (0.3) b | 6.8 (0.1) ab |
