Table 5 Details of LCMS conditions used for quantification of ectoine.
Sr. no. | LC parameter | Sr. no. | QTOF parameter | ||
|---|---|---|---|---|---|
1 | Mobile phase | 1 | Ion-polarity | Positive MS mode | |
(i) | A | Water with formic acid | |||
(ii) | B | Methanol with formic acid | 2 | Source | Dual AJS ESI |
2 | Flow | 1 ml min−1 | 3 | Gas Temp | 350 °C |
3 | Time (min) | B | 4 | Drying GAS | 10 l min−1 |
(i) | 0 | 10 | 5 | Nebulizer | 40 psi |
(ii) | 10 | 50 | 6 | Sheath Gas Temperature | 300 °C |
(iii) | 11 | 10 | 7 | Sheat Gas Flow | 12 l min−1 |
(iv) | 13 | 10 | 8 | V cap | 4000 V |
4 | Injection volm | 20 μl | 9 | Fragmentor | 150 V |
5 | Total run time | 13 min | 10 | Skimmer | 65 V |
6 | Column Temperature | 40 °C | |||
