Figure 5
From: ACLS4 could be a potential therapeutic target for severe acute pancreatitis
Severe Acute pancreatitis upregulates ACSL4. Mice were injected with cerulein or arginine to induce severe acute pancreatitis. (A) qPCR of ACSL4, IL1R1, GALNT3 and WSB1 mRNA levels in pancreas (n = 5). (B,C) (B) IHC images of pancreas section and ACSL4 protein were stained. (C) Quantification of ACSL4 positive area (n = 3). (D,E) (D) Western blot of ACSL4 and GAPDH in pancreas. Original blots are presented in Supplementary Fig. 1. (E) Quantification of ACSL4 protein. The protein level of ACSL4 was normalized to those of GAPDH (n = 3). P values were determined by unpaired two-tailed Student’s t-test; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.
