Figure 3 | Scientific Reports

Figure 3

From: Glycopolymer binds pathogenic IgM autoantibodies and pulls them into the mononuclear phagocyte system for degradation

Figure 3

Transmission electron microscopy (TEM) images of PPSGG and the anti-HNK-1 IgM antibody with the lower panels displaying the squared areas of the images. The size and shape of PPSGG (A), anti-HNK-1 IgM (B), and the PPSGG/anti-HNK-1 IgM complex (C) were characterized by TEM. PPSGG and anti-HNK-1 IgM samples were incubated on a Copper grid 400 mesh coated with Parlodion and carbon, blotted and washed with water, and stained with 2% uranylacetate. Images were taken with a Tecnai G2 Spirit instrument (120 kV TEM Microscope with EMSIS Morada camera). PPSGG has a size of approximately 100 nm, IgM is pentameric with a diameter of 40–50 nm, and the formed complexes are of similar size as the antibody. No crosslinking or large aggregate formation was observed.

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