Figure 3 | Scientific Reports

Figure 3

From: Heat shock protein 70 kDa (HSP70) is involved in the maintenance of pig sperm function throughout liquid storage at 17 °C

Figure 3

Effects of HSP70 activity inhibition during liquid storage of pig semen at 17 °C on (a) ratio of intensity between JC-1agg and JC-1mon; (b) intracellular Ca2+ levels (Fluo3+) in viable sperm (PI−); (c) intracellular superoxide levels (E+) in viable sperm (Yo-Pro-1−); and (d) intracellular total ROS levels (DCF+) in viable sperm (PI−). Different letters indicate significant differences (P ≤ 0.05) between experimental groups (0, 0.05, 0.1 and 0.2 μM of YM-1) on a given time point (0, 4, 10, 14 and 21 days). Results are expressed as the mean ± SEM (N = 6).

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