Figure 1
From: Repetitive invasive lung function maneuvers do not accentuate experimental fibrosis in mice
Repeated lung function measurement does not cause weight loss or inflammation in mice. (A) Experimental setup. Mice of the single lung function (1 × LFT) group were exposed to AdCL or AdTGF-β1 (1 × 108 PFU/mouse) and received additional anesthesia at days 14 and 21. On day 28, mice underwent LFT and were analyzed on day 29 post-treatment. Mice in the 4 × LFT group underwent lung function tests at day 0 followed by administration of AdCL or AdTGF-β1 (1 × 108 PFU/mouse). Lung function was measured at days 14, 21 and 28 followed by endpoint analysis at day 29. (B) Body weight of AdTGF-β1 treated mice with one LFT at day 28 (black line) or four LFT (red line). (C–F) Resident alveolar macrophages (rAM; C), exudate macrophages (ExMacs; D), lymphocytes (E) and neutrophils (F) in BAL fluid of mice exposed to AdCL (white bars) or AdTGF-β1 (black bars) after one or four LFT, as indicated. Data are presented as mean ± SD of n = 8 mice per group and time point and are representative of two independent experiments. *p < 0.05, **p < 0.01 compared to disease control (Mann–Whitney U test).
