Figure 3
From: Repetitive invasive lung function maneuvers do not accentuate experimental fibrosis in mice
Repeated lung function maneuvers do not affect developing lung fibrosis in mice. (A–F) Histopathology of mice exposed to AdCL (A), or AdTGF-β1 (C, E) with one LFT at day 28 or mice exposed to AdCL (B), or AdTGF-β1 (D, F) with four LFT at day 0, 14, 21 and 28. Lung sections were stained with hematoxylin/eosin (HE) (A–D) or Elastica van Gieson (EvG) (E, F) for assessment of lung tissue remodeling. Histopathology was performed on day 29 post-treatment. Closed arrows, lymphoplasmacellular infiltrates; open arrows, widened alveolar septa; asterisk, bronchus (original magnification, × 10; scale bar, 100 µm). (G) Lung collagen content in AdCL (white bars) or AdTGF-β1 (black bars) exposed mice on day 28 after single or repeated LFT, as indicated. Data are presented as mean ± SD of n = 5 mice per group and time point and are representative of two independent experiments. **p < 0.01 compared to disease control (Mann–Whitney U test).
