Figure 4

Repeated lung function maneuvers do not affect leukocyte subset compositions in lungs of mice. (A–C) Immunophenotypic analysis of lymphocytes (A), macrophages (B) and neutrophils (C) in lung parenchyma of AdTGF-β1-exposed mice after 4 × LFT (day 29), as detailed in Materials and Methods. (D–G) FACS analysis of CD3⁺ T cells (D), CD4⁺ T cells (E), CD8⁺ T cells (F) and CD19⁺ B cells (G) in BAL fluid of mice after AdCL (white bar) or AdTGF-β1 (black bar) treatment with single or repeated LFT. (H–K) FACS analysis of CD3⁺ T cells (H), CD4⁺ T cells (I), CD8⁺ T cells (J) and CD19⁺ B cells (K) in lungs of mice after AdCL (white bar) or AdTGF-β1 (black bar) treatment with single or repeated LFT. (L–M) Neutrophils in BAL (L) and lung (M) in mice exposed to AdCL (white bar) or AdTGF-β1 (black bar) with single or repeated LFT. (N–O) Resident alveolar macrophages (rAM; N) and ExMacs (O) in mice exposed to AdCL (white bar) or AdTGF-β1 (black bar) with single or repeated LFT, as indicated. Data are shown as mean ± SD of n = 8 mice per time point and experimental group and are representative of two independent experiments. **p < 0.01, ***p < 0.001 compared to disease control or the single LFT group, as indicated (Mann–Whitney U test).