Figure 5



Pathway enrichment and flux analysis reveals that the TCA and urea cycles in the liver and kidney are impacted 24 h after AKI. (A) Pathway diagram including tricarboxylic acid (TCA) and urea cycles, illustrating steady state and labeled carbon study results in liver. Metabolites higher (red) or lower (blue) in AKI versus Sham mouse livers at 24 h post-procedure. Analytes in gray were detectable but not significantly different in AKI versus Sham. Analytes in black were not detectable through metabolomic analysis. Differences found in labeled carbon studies are designated by §. (B) Labeled carbon study results for TCA Intermediates in liver and kidney. (C) Labeled carbon study results for TCA-related Amino Acids in liver and kidney. For (B) and (C), y-axes represent peak areas (arbitrary units). For both liver and kidney, n = 8 Sham, n = 8 AKI. P-value < 0.05 (*) in Sham vs AKI. Data can be found in Supplementary Tables S6 and S7, respectively.