Figure 4


(a) The Neighbor-joining tree showing the genetic connections of 388 complete PiCV genome sequences investigated in this study. The sequences were encoded with the following formula: B (blood)/S (swab), loft number (I–V), _pigeon number (1–3), sampling date (a–h), _clone number (C1–10) and the Accession Number. The color marking of individual sequences indicates the loft of origin: green—loft I, blue—loft II, purple—loft III, pink—loft IV and orange—loft V. Sequences belonging to different genotypes are highlighted with different shades of grey. (b) PiCV genotypes recognized in the investigated pigeons during the experiment. Lack of symbols near the pigeon shape indicates an unsuccessful recovery of the complete PiCV genome on the specific sampling date. Digits near the selected shapes indicate the number of PiCV clones belonging to the specific genotype (G I–G XIII) detected in blood samples (red digits) and swab samples (blue digits) taken from the experimental birds. The pigeon lofts are encoded by color according to Fig. 1.