Figure 4
Bergenin influences migration and angiogenesis in cervical cancer cells. (A) Scratch assay was performed in SiHa cells with or without treatment of bergenin(100 µM and 200 µM) for 24 h. Pictures were taken at the start of the experiment (0 h) and 24 h later. Images of representative experiments are shown, and the means ± SD are illustrated as graphical representation. (B) Wound scratch assay was performed in C33A cells with or without treatment of bergenin (100 µM and 200 µM) for 24 h. Pictures were taken at the start of the experiment (0 h) and 24 h later. (C) Representative images from Transwell migration assay upon treatment of bergenin (100 μM and 200 μM) for 48 h in SiHa cells and means ± SD are illustrated as graphical representation. (D) Western blot of indicated markers of EMT and pathways after 100 μM and 200 μM bergenin treatment for 48 h in SiHa and C33A cell lines. The blots were cut before being subjected to hybridization with antibodies in the blotting procedure, and the original blots are provided in the supplementary information.
