Figure 3

BCHE triggered ferroptosis by inducing intracellular iron and lipid peroxide accumulation in human BC cells. (a) Intracellular Fe²⁺ levels in BCHE-treated human BC cells were detected by flow cytometry. (b) Laser confocal microscopy was used to observe changes in intracellular Fe²⁺ levels in human BC cells after BCHE treatment (MCF-7, 40 µg/mL; MDA-MB-231, 20 µg/mL). The scale bar represents 25 µm. (c) A Fe²⁺ detection kit was used to measure the intracellular Fe²⁺ content in human BC cells. (d) Intracellular ROS content in BCHE-treated BC cells was detected via flow cytometry. (e) The levels of intracellular lipid peroxide in human BC cells following BCHE treatment were detected by flow cytometry. (f) Laser confocal microscopy was used to observe changes in intracellular lipid peroxide levels in human BC cells after BCHE treatment (MCF-7, 40 µg/mL; MDA-MB-231, 20 µg/mL). Green: FITC filter; Red: Texas Red filter; Scale bar represents 25 µm. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001.