Figure 8
Effects of sulfated- SK and dsRNA-mediated knockdown of BNGR-A9 on feeding behavior of silkworm. (A) Effect of Bombyx sulfated- SK on silkworm body weight. Larvae were injected with BmsSK (final concentration 10 nM) or an equivalent volume of HBM every day since the second day of 5th instar (Day 0), and body weight was measured every 2 days. (B) Effects of dsRNA injection on bngr-a9 expression in Midgut. BNGR-A9 dsRNA (5 μg) or EGFP dsRNA (5 μg) were injected into larvae of silkworm every other day. bngr-a9 gene expression level in treated silkworm larvae was determined by qRT-PCR as described in Materials and Methods. The data were displayed as fold changes relative to the first-day expression level. (C) Effects of BNGR-A9 dsRNA and sulfated-SK on average food intake of silkworm. 24 h after dsRNA injection, synthesized BmsSK (final concentration 10 nM) or HBM were injected into the larvae of silkworm. Food consumption of silkworms was measured every 2 days. (D) Effect of dsRNA and sulfated- SK on the trehalose level in the hemolymph. 24 h after dsRNA injection, silkworm larvae were injected with Bombyx sulfated- SK (final 10 nM) or the same volume of HBM, then hemolymph was collection 24 h later. All data were taken from at least three independent experiments, and data were analyzed using a Student’s t-test (*p < 0.05).
