Figure 4 | Scientific Reports

Figure 4

From: Lower urinary dysfunction as a long-term effect of childhood vincristine treatment, with potential influences by sex and dose

Figure 4

In vitro physiological evaluation of the bladder strips. (A) Experimental timeline. (B) Peak contractile force in response to electric field stimulation (EFS, left), carbachol (CCh, center), and substance P (SP), ABMA, and KCl (right). The force was normalized with tissue weight. (C) Component analysis in contraction evoked by EFS (32 Hz). Black, open, and grey bars represent the purinergic (ABMA-sensitive), muscarinic (atropine-sensitive), and non-purinergic, non-muscarinic (ABMA and atropine-insensitive) component, respectively. The data were expressed as a percentage of the response in absence of inhibitors. (D) Peak contractile force normalized to the response to KCl. EFS (left), CCh (center), and SP and ABMA (right) [N = 6–8 mice per group]. The data were expressed as fold of the peak force in response to KCl. Blue and orange lines and squares represent the control and VCR group, respectively (B,D). Mean ± SEM, *p < 0.05, #p < 0.005 vs. the control group.

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