Figure 6

Gene expression analyses. The level of mRNA expression of each gene in the bladder (A) and Ls-DRG (B) is expressed as the fold difference to that in the control group. (A) Normalized with a mean value of Gapdh and Tbp. [N = 5–6 per group]. (B) Normalized with Gapdh and Pgk1. [N = 6–7 per group]. Blue and orange bars represent the control and VCR groups, respectively. Mean ± SEM. *p < 0.05 vs. the control group. (C) Representative Western blotting result with the antibodies against Kit (top) and β-actin (Actb, bottom). Figures were prepared using Adobe Photoshop CS6 and Microsoft Excel (https://office.microsoft.com/excel).