Figure 5 | Scientific Reports

Figure 5

From: Increased biofilm formation in dual-strain compared to single-strain communities of Cutibacterium acnes

Figure 5

Confocal images and spatial analysis of the dual-type biofilm of C. acnes. Fluorescence in-situ hybridization was applied using strain-specific probes. Yellow, strain EASDk81A; purple, strain EASDk81B. (A) 3D visualization of z-stack images taken through the biofilm from top to bottom of the disc. Left, separate images for each probe; right, overlay of both channels. (B) Perpendicular xy-, xz-, and yz-planes through randomly selected points in the biofilm. XY image is 160 × 160 µm and shown at 15 µm depth in the biofilm. (C). Spatial analysis of the dual-type biofilm. The biofilms were created in triplicates and z-stack images were analyzed, resulting in relative abundances of EASDk81A (yellow) and EASDk81B (purple). Statistical analysis utilized R package geom_smooth, and error envelopes are displayed for both strains.

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