Figure 1 | Scientific Reports

Figure 1

From: miR-124-3p downregulates EGR1 to suppress ischemia-hypoxia reperfusion injury in human iPS cell-derived cardiomyocytes

Figure 1

IH/R increases the expression of EGR1 and induces apoptosis in hiPSC-CMs. (A) A chart of the experiments of the induction of IH/R in hiPSC-CMs. (B) Representative immunofluorescence staining for cardiac isoform of troponin-T (cTnT) for hiPSC-CMs. (C) qRT-PCR of human EGR1 and RPLP1 (endogenous control) for hiPSC-CMs (n = 6). **P < 0.01. (D,E). Western blotting of human EGR1 and GAPDH (endogenous control) (n = 9). ****P < 0.0001. (F,G). Immunofluorescence staining for cleaved Caspase-3 (red), cTnT (green) and DAPI (blue) (n = 42). Scale bars 100 μm. ***P < 0.001. (H). Western blotting of human BCL2, BAX and GAPDH (endogenous control) (n = 6). (I). The ratio of BCL2/BAX in Control group and IH/R group, **P < 0.01.

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