Figure 4
From: Exposure to Gulf war illness-related chemicals exacerbates alcohol-induced liver damage in rodents
Exposure of mice to GWI-related chemicals PER and PB, induced chronic, low-grade hepatic fibrosis in mice. (A) Changes in mRNA expression of genes with role in fibrogenesis, i.e., CTGF, PDGFβ, TGFβ1 in livers from Naïve and GWI mice. (B) Representative images of Sirius Red staining of liver sections. (C) Quantification of proteins that are markers of liver fibrosis, based on image analysis of Sirius Red staining in livers from Naïve and GWI mice. IHC images (D) and quantification by image analysis (E) was performed for αSMA protein that is a biomarker of activated hepatic stellate cells. The intrahepatic bile duct mass in livers of GWI vs Naïve mice, was measured by IHC of CK19 marker of cholangiocytes; representative images (F) and quantification (G) are shown. N = 4, p < 0.05. #, GWI vs Naïve mice. Scale bar, 50 µm.
