Figure 5
From: Exposure to Gulf war illness-related chemicals exacerbates alcohol-induced liver damage in rodents
The effects of PER and PB on HepG2 cells in vitro. HepG2 cells were treated with PER, PB or a combination of PER and PB as described under Methods. Changes induced by these treatments in mRNA’s encoding for PLIN2 (A), PNPLA3 (B) and SREBP1 (C), were measured using RT-qPCR. The influence of PER, PB or PER + PB on lipid accumulation in HepG2 cells, was assessed by staining of the lipids with Nile Red: images of Nile Red-labeled lipid droplets (D) and quantification of area percent staining (E) are shown. N = 4, p < 0.05. #, PER, PB, PER + PB vs Vehicle. Scale bar, 20 µm.
