Figure 8
From: Exposure to Gulf war illness-related chemicals exacerbates alcohol-induced liver damage in rodents
Influence of EtOH on liver fibrosis and intrahepatic biliary duct mass in GWI-mice vs. Naïve controls. Liver sections from GWI and Naïve mice that consumed EtOH vs mice that didn’t consume EtOH, were assessed for fibrosis using Sirius Red staining of ECM proteins and αSMA IHC. (A) Representative images of Sirius Red-staining. (B) Quantification of % area stained measured by image analysis. Images of αSMA IHC, and quantification data based on image analysis are shown in panels (C) and (D), respectively. Changes in IBDM were assessed by CK19 IHC: (E) representative images; (F) quantification of CK19 based on image analysis. N = 4, p < 0.05. #, GWI vs Naïve mice. $, EtOH vs no EtOH. Scale bar, 50 µm.
