Figure 9

Depletion of macrophages improved liver function, and reduced hepatic inflammation caused by EtOH consumption in GWI mice. Serum transaminases ALT (A) and AST (B), as well as images of H&E staining (C) in Naïve or GWI mice that were treated with vehicle, or PLX3397 before EtOH binging (i.e., PLX, + EtOH), are shown. IHC images of Clec4f marker of Kupffer cells, and quantification data using image analysis are illustrated in panels (D) and (E), respectively. CD11b marker of infiltrated monocyte-derived macrophages in the liver was measured by IHC (F) and quantification using image analysis (G). Changes in Clec4f and CD11b mRNA were measured in livers from GWI mice when treated with vehicle, or PLX3397 prior to EtOH (H). Relative expression of mRNA for IL-1β, IL-6, CCL2 and TNFα was measured in the same groups of mice (I). N = 4, p < 0.05. #, EtOH vs vehicle. $, PLX + EtOH vs EtOH. Scale bar, 50 µm.