Figure 3

Excessive autophagy signaling following 24 h of starvation in PLK2-KO female mice. (A) Gravimetric analysis of heart weight (HW), body weight (BW), and the HW/BW ratio (n = 6–12 mice in each group) in female mice subjected to 24 h of starvation. (B). Representative example of WB results after 24 h of starvation for LC3I, LC3II, p62, and GAPDH with quantification (C). Autophagosome accumulation was calculated as the fold of starvation to untreated (fed) mice in each group (inset). (D) Mice were subjected to 24 h of starvation followed by 3 h of CQ (80 mg/kg). Representative example of WB results (left panel) with quantification (right panel). Autophagy flux was calculated as the delta between starvation + CQ and CQ in each group (∆ ± starvation, inset). (E) Representative example of WB results for phosphorylated AKT⁴⁷³ (pAKT), total AKT (t-AKT), and GAPDH and quantification of pAKT⁴⁷³ relative to GAPDH (n = 5–7 mice in each group). Statistical analyses were conducted with GraphPad Prism 6 as described in the Methods. *p < 0.05 and **p < 0.01.