Figure 2 | Scientific Reports

Figure 2

From: Delivery of CDNF by AAV-mediated gene transfer protects dopamine neurons and regulates ER stress and inflammation in an acute MPTP mouse model of Parkinson’s disease

Figure 2

hCDNF alleviates motor function impairments and improves gait dynamics in an acute MPTP mouse model of PD. (A) Diagram of the experimental design. C57BL/6 mice received bilateral intrastriatal injections of AAV-GFP, AAV-hCDNF, or PBS. Four weeks later, the mice were intraperitoneally injected with PBS or MPTP four times per day at 2 h intervals. The mice were sacrificed 7 days after the last MPTP injection. Before the mice were sacrificed, behavioural tests (the rotarod test, coat hanger test, and open field test and DigiGait analysis) were performed at 5 and 6 days after MPTP injection. (B) Illustration of the coat hanger test. (C) The latency to fall off the rotarod was recorded. (D) Evaluation of motor score in the coat hanger test. (E) The latency to fall off the coat hanger was recorded. (F) The time to reach zone A in the coat hanger test was recorded. (G) Schematic diagram of gait data (left) and paw print data (right) from DigiGait analysis. The gait of each mouse on a transparent treadmill was recorded by a digital camera while the mouse walked. One stride consisted of a stance phase (the time during which the paw contacted the belt) and swing phase (the time during which the paw did not contact the belt). One stance phase had two subphases: brake and propulsion. Brake was the first subphase of the stance phase and was defined as the initial paw contact to maximum paw contact after commencing the swing phase. Propulsion was defined as the duration of maximum paw contact just before the swing phase. Additionally, stride length, width and paw angle were analysed from the paw print data. (H) Stride length variability. (C) n = 13 for the PBS group, n = 13 for the PBS + MPTP group, n = 12 for the AAV-GFP + MPTP; AAV-GFP + MPTP group, and n = 13 for the AAV-hCDNF + MPTP group. (D–F) n = 6 for the PBS group, n = 7 for the PBS + MPTP group, n = 5 for the AAV-GFP + MPTP group, and n = 6 for the AAV-hCDNF + MPTP group. (H) n = 12 for the PBS group, n = 12 for the PBS + MPTP group, n = 12 for the AAV-GFP + MPTP group, and n = 12 for the AAV-hCDNF + MPTP group. One-way ANOVA followed by Tukey’s multiple comparisons post hoc test. All the data are expressed as the mean ± S.E.M. *p < 0.05, **p < 0.01, and ***p < 0.001. n.s.: not significant.

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