Figure 5

hCDNF decreases astroglial IL-1β and C3 expression in the acute MPTP model. (A,B) Z-stack images of immunofluorescence double staining for GFAP (red), IL-1β (cyan), DAPI (blue) and GFP or hCDNF (green) in the STR (upper panel, A) and SN (lower panel, B). The dotted white rectangles indicate the enlarged areas. Scale bar: 50 µm for the z-stack images and 20 µm for the enlarged images. (C) Quantification of the GFAP + IL-1β pixel area in the STR and SN. (D,E) Z-stack images of immunofluorescence double staining for GFAP (red), C3 (cyan), DAPI (blue) and GFP or hCDNF (green) in the STR (upper panel, C) and SN (lower panel, D). The dotted white rectangles indicate the enlarged areas. Scale bar: 50 µm for the z-stack images and 25 µm for the enlarged images. (F) Quantification of the GFAP + C3 pixel area in the STR and SN. (C) STR: n = 16 images/4 mice for the PBS group, n = 14 images/4 mice for the PBS + MPTP group, n = 15 images/4 mice for the AAV-GFP + MPTP group, and n = 16 images/4 mice for the AAV-hCDNF + MPTP group; SN: n = 16 images/4 mice for the PBS group, n = 16 images/4 mice for the PBS + MPTP group, n = 16 images/4 mice for the AAV-GFP + MPTP group, and n = 16 images/4 mice for the AAV-hCDNF + MPTP group. (F) STR: n = 15 images/4 mice for the PBS group, n = 13 images/4 mice for the PBS + MPTP group, n = 12 images/4 mice for the AAV-GFP + MPTP group, and n = 13 images/4 mice for the AAV-hCDNF + MPTP group; SN: n = 15 images/4 mice for the PBS group, n = 13 images/4 mice for the PBS + MPTP group, n = 14 images/4 mice for the AAV-GFP + MPTP group, and n = 12 images/4 mice for the AAV-hCDNF + MPTP group. (C,F) One-way ANOVA followed by Tukey’s multiple comparisons post hoc test . All the data are expressed as the mean ± S.E.M. *p < 0.05, **p < 0.01, and ***p < 0.001.