Figure 4 | Scientific Reports

Figure 4

From: Biogenic amine tryptamine in human vaginal probiotic isolates mediates matrix inhibition and thwarts uropathogenic E. coli biofilm

Figure 4

Screening and Characterization of Cell free supernatant from Lactobacillus sp. C1–C7 are Compounds 1 to Compound 7 (A) visualization of column chromatography purified fractions of CFS in TLC (B) TLC plate after UV exposure where C2 shows fluorescence marked by dotted yellow line (C) Screening of the C1 to C7 fractions for matrix inhibition, treatment with C2 fraction (marked in square) causes biofilm matrix inhibition, E. coli UTI89 colony biofilm phenotype was analyzed by inoculating it as a spot containing the metabolites on congo red media. Red, Dry and Rough (RDAR) colony morphology indicate matrix production, whereas Smooth and White (SAW) colony morphology indicate absence of matrix production (D) Crystal Violet assay showing ability of fractions to inhibit biofilm formation, mean ± SD from 3 replicates plotted for all panels. (E) GC–MS of C2.

Back to article page