Figure 1:
12% SDS-PAGE revealing the purification of Ha006a protein by employing chromatography techniques. In both the gels, Ladder: Protein Ladder (Thermo Scientific) was loaded. (a) Purification was performed using the Ni-NTA affinity chromatography technique. The gel bears witness to a gradient of imidazole, identified as follows: First wash with 5 mM imidazole, second with 10 mM, Third with 15 mM, then with 20 mM, and the elution with 250 mM imidazole. (b) Purification was performed by anion exchange chromatography. In the gel, supernatant signifies the flow-through obtained as the initial passage through the column and the elution reveals the purified Ha006a protein, achieved with a buffer containing 300 mM NaCl. The original gel images are available in the supplementary file (Fig. S1 and S2).
