Figure 4
From: Intense impact of IL-1β expressing inflammatory macrophages in acute aortic dissection
IL-1β+ inflammatory macrophages are accumulated before the onset of macroscopic aortic dissection (AD) in mice. (A) Re-clustering of monocytes and macrophages into three groups: mouse aortas without Ang II or BAPN (control), mouse aortas without AD after exposure to Ang II and BAPN (non-AD), and mouse aortas with AD after exposure to Ang II and BAPN (AD). (B) Violin plots depicting single-cell gene expression of each canonical monocyte or macrophage marker for clustering. (C) Proportion of each monocyte-macrophage cluster. (D) Heatmap of the top-5 differentially expressed genes in each monocyte and macrophage cluster. (E) Trajectory pseudo-time analysis in Monocle3 with Seurat cluster annotations (left) and change in the expression of IL-1β across pseudo-time for the monocyte and monocyte-derived macrophage partitions (clusters 0, 1, 2, 3, 4) in the non-AD and AD groups (right). Macs macrophages, Monos monocytes, Ang II Angiotensin II, BAPN β-aminopropionitrile.
