Figure 4

Epitope mapping of 2D6-2C6 on tau. (A) Schematic representation of human wild-type (WT) tau protein and its deletion mutants. The full-length 2N4R tau consists of an N-terminal region that includes a proline-rich region and N-terminal inserts, a microtubule binding repeat region, and a C-terminal region. The ΔN mutant lacks residues 1–243, the ΔR mutant lacks residues 244–369, and the ΔC mutant lacks residues 370–441. (B) cDNAs encoding WT, ΔN, ΔR and ΔC were transfected into cultured COS-7 cells. Tau proteins in the cell lysate were detected with the pan-tau antibody JM (B, left panel) and 2D6-2C6 (B, right panel) using SDS‒PAGE western blot analysis. (C) Schematic representation of peptides with partial sequences of the C-terminal region. (D–H) The peptides were dotted onto a nitrocellulose membrane and then reacted with 2D6-2C6.