Figure 1
From: Biparatopic anti-PCSK9 antibody enhances the LDL-uptake in HepG2 cells
Phage-ELISA assay, SDS-PAGE and affinity tests of two novel sdAbs. (A) The phage-ELISA results. The horizontal axis represents different samples in 96 well plate. The vertical axis represents the OD450 value. ‘(Blank)’ refers to the PBS control (A1–A3 wells) of the ELISA assay. ‘(No coating)’ refers to the no-PCSK9 coating negative control (H10–H12 wells) of the ELISA assay. The star (#) represents positive (the OD450 ratio of the experimental group/blank group ≥ 2.1). (B) LaneM: the protein marker (Fermentas, USA). Left lane: the VHH-D8 antibody (~ 17 kDa) expressed by HB2151 E. coli (pMECS-D8); Right lane: the VHH-H2 antibody (~ 17 kDa) expressed by HB2151 E. coli (pMECS-H2); (C) Affinity determination between VHH-H2 and human PCSK9-his. (D) Affinity determination between VHH-D8 and human PCSK9-his. The horizontal axis represents the timeline. The vertical axis represents the relative response unit. (E) A list of the main properties of the screened sdAbs (VHH-H2 and VHH-D8).
