Figure 3
From: Biparatopic anti-PCSK9 antibody enhances the LDL-uptake in HepG2 cells
Schematic representation, SDS-PAGE and SPR tests of B11-H2-Fc. (A, B) Schematic representation of two VHHs and biparatopic B11-H2-Fc configurations. “(G4S)3” is a 15- amino acid peptide linker (GGGGSGGGGSGGGGS). “IgG1-hinge linker” means the hinge region between the human IgG1 Fab and Fc. (C) Lane M: the protein marker (Fermentas, USA). Lane 1: the VHH-B11-Fc antibody (~ 45 kDa) expressed by HEK293F (pFUSE-hIgG1-B11); Lane 2: the VHH-H2-Fc antibody (~ 45 kDa) expressed by HEK293F (pFUSE-hIgG1-H2); Lane 3: the B11-H2-Fc antibody (~ 65 kDa) expressed by HEK293F (pFUSE-hIgG1-B11-H2) under reducing conditions. (D) SPR tests between B11-H2-Fc and human PCSK9-his. The horizontal axis represents the timeline. The vertical axis represents the relative response unit.
