Figure 5 | Scientific Reports

Figure 5

From: The role and mechanism of action of miR-483-3p in mediating the effects of IGF-1 on human renal tubular epithelial cells induced by high glucose

Figure 5

The study employed dual-luciferase reporter gene assays and real-time quantitative PCR to investigate the regulatory correlation between miR-483-3p and IGF1. The findings revealed that the miR-483-3p level in the HG + mimics group was significantly higher than in the control group and the HG group (P < 0.05), while IGF1 was significantly lower than in the control group and the HG group (P < 0.05). In the HG + inhibitor group, the miR-483-3p level was significantly lower than in the HG group (P < 0.05), and IGF1 was significantly higher than in the HG group (P < 0.05) (A,B). The dual-luciferase reporter gene results confirmed a negative regulatory relationship between miR-483-3p and IGF1 (P < 0.05) (C,D). These data suggest that miR-483-3p and IGF1 exhibit a targeted negative regulatory relationship.

Back to article page