Table 1 The information of primer sets for qPCR.

From: The role and mechanism of action of miR-483-3p in mediating the effects of IGF-1 on human renal tubular epithelial cells induced by high glucose

Gene

Primer

Sequence (5′-3′)

Homo GAPDH

Forward

TCAAGAAGGTGGTGAAGCAGG

Reverse

TCAAAGGTGGAGGAGTGGGT

U6

Forward

CGCTTCGGCAGCACATATAC

Reverse

AAATATGGAACGCTTCACGA

miR-483-3p

loop primer

GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAAGACGGG

F primer

TGCGCTCACTCCTCTCCTCCC

Gene

Primer

Sequence (5′-3′)

Homo GAPDH

Forward

TCAAGAAGGTGGTGAAGCAGG

Reverse

TCAAAGGTGGAGGAGTGGGT

Homo IGF1

Forward

TCATGCCTTGGTCTCCTTGT

Reverse

TGCTTTGATGGTCAGGTTGC

Homo Bax

Forward

AAGAAGCTGAGCGAGTGTCT

Reverse

GTTCTGATCAGTTCCGGCAC

Homo bcl-2

Forward

TGTGGAGAGCGTCAACCG

Reverse

AGACAGCCAGGAGAAATCAAA

Homo caspase-3

Forward

AGAGGGGATCGTTGTAGAAGTC

Reverse

ACAGTCCAGTTCTGTACCACG

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