Figure 7
SARS-CoV-2 [S1S2] spike induces proteolytic activation of α and γ ENAC in differentiated BCi-NS1.1 (d-BCi) epithelia. ALI differentiated dBCi cells were incubated apically with different concentrations of Wuhan-Hu-1 [S1S2] spike protein for 4 h, washed and then incubated for additional 20 h under ALI conditions. (a) A representative Western blot image showing the detecting levels of total γ ENaC and two proteolytic cleavage products (red arrowheads). (b) A representative Western blot image showing the detecting levels of total α ENaC and its proteolytic cleavage product (red arrowhead). β-actin was used for equal loading of protein. Western blots are representative of the results from three independent experiments.
