Figure 4 | Scientific Reports

Figure 4

From: Evidence of membranolytic targeting and intracellular citrullination in neutrophils isolated from patients with rheumatoid arthritis

Figure 4

Citrullination during programmed cell death. (a) A neutrophil treated with perforin with activated caspase 3 and citrullination. (b) Neutrophils in NETosis after treatment with 100 nM phorbol ester. (c) Four neutrophils undergoing apoptosis after treatment with 1 µM staurosporine and stained for activated caspase 3 and citrullination. The last neutrophil (bottom row) is not in apoptosis. (d) Neutrophils in various stages of pyroptosis after treatment with 10 µM nigericin and stained with anti-ASC and ACPA mAb L201_10D07. Note the morphology of the cells and the faint ACPA staining in the third cell. (e) anti-modified citrulline immunoblot of lysates from neutrophils treated as indicated. (f) neutrophils in late-stage pyroptosis.

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