Figure 4 | Scientific Reports

Figure 4

From: Selection and characterization of human scFvs targeting the SARS-CoV-2 nucleocapsid protein isolated from antibody libraries of COVID-19 patients

Figure 4

Co-immunoprecipitation of SARS-CoV-2 viral N protein with anti-N scFvs expressed in Vero-TMPRSS2 cells. Every panel displays three types of samples: (i) control cells not transfected and not infected (wt), ii) cells transfected with the scFv and (iii) cells both transfected with the scFv and infected with SARS-CoV-2 B.1 strain. For each sample the input for total cell lysate (I) and the co-immunoprecipitated proteins (co-IP) were loaded. (A): Co-immunoprecipitation of unrelated scFv (#58F) used as negative control. (B): Co-immunoprecipitation of scFv#66. (C): Co-immunoprecipitation of scFv#93. (D): Co-immunoprecipitation of scFv#255. (E): Co-immunoprecipitation of scFv#291. (F): Co-immunoprecipitation of scFv#336. Immunoprecipitated scFvs were detected using anti-HA antibody and co-immunoprecipitated N protein was identified using an anti-N antibody. Actin was used as loading control. Uncropped blots are presented in the Supplementary material section “Images of the original blots”.

Back to article page