Table 2 Yield and aggregation propensity of scFvs expressed in E. coli.

From: Selection and characterization of human scFvs targeting the SARS-CoV-2 nucleocapsid protein isolated from antibody libraries of COVID-19 patients

scFv (pI)

Yield pre-SEC (mg/L)

Monomeric form obtained by SEC

Notes

#5 (8.48)

0.24 mg/L

NO

High tendency to form oligomers

#24 (9.21)

10.79 mg/L

Yes

 

#77 (7.72)

0.04 mg/L

NA

Low yield

#93 (6.22)

13.06 mg/L

NO

High tendency to form oligomers

#255 (6.70)

1.175 mg/L

NO

High tendency to form oligomers

#261 (8.58)

0.045 mg/L

NA

Protein precipitation after refolding

#291 (8.50)

0.142 mg/L

NO

High tendency to form oligomers

#336 (7.72)

2.44 mg/L

Yes

 
  1. SEC: size exclusion chromatography; yield preSEC: yield of total scFv protein, before SEC.
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