Table 3 Binding parameters for the interaction between the recombinant N protein and scFvs #24 and #336.

From: Selection and characterization of human scFvs targeting the SARS-CoV-2 nucleocapsid protein isolated from antibody libraries of COVID-19 patients

Analyte

Ligand

KD(nM)

KD Error (nM)

ka(1/Ms)

ka Error

kd (1/s)

kd Error

Full R2

scFv #24

N-FL

27.8

0.0

7.64E + 04

1.03E + 0.2

2.13E-0.3

2.04E-0.6

0.9976

N-CTD

74.9

0.2

8.35E + 03

1.71E + 0.1

6.25E-0.4

1.30E-0.6

0.9994

AT-N-CTD

66.3

0.2

8.27E + 0.3

1.70E + 0.1

5.48E-0.4

1.28E-0.6

0.9994

scFv #336

N-FL

196

1.6

6.42E + 0.4

4.53E + 0.2

1.26E-0.2

5.28E-0.5

0.9557

N-CTD

95.1

0.4

5.74E + 0.4

2.10E + 0.2

5.46E-0.3

5.28E-0.5

0.9944

AT-N-CTD

69.4

0.3

5.58E + 0.4

2.02E + 0.2

3.88E-0.3

6.49E-0.6

0.9924

  1. ka and kd values were estimated by globally fitting the BLI response intensity (nm) as a function of scFv concentration (nM) with the Octet Data Analysis Software, using a 1:1 Langmuir binding model. KD was calculated as kd/ka. Errors for ka and kd are reported as standard errors of the mean calculated from the fitting curves.
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