Figure 2 | Scientific Reports

Figure 2

From: Development of a robust and generalizable algorithm "gQuant" for accurate normalizer gene selection in qRT-PCR analysis

Figure 2

(A) Ct value distribution for genes, depicted as a box plot with a strip plot overlay. Cycle threshold (Ct) values for miRNA expression 'Ct Mean (let-7c-5p)', 'miR-16-5p', 'miR-30a-5p', 'miR-30d-5p', and 'miR-10b-5p' are distributed as shown in this figure. Genes are plotted on the x-axis, and their corresponding Ct values are quantified on the y-axis. The interquartile range (depicted by boxplot), median (depicted by coloured line in boxplot), mean (depicted by red dashed line) and outliers are displayed in each box plot, and the strip plot offers more information on the distribution of the data. (B) KDE Plot of Ct Values Across Genes. A Kernel Density Estimation (KDE) plot of cycle threshold (Ct) values for the genes "Ct Mean (let-7c-5p)", "miR-16-5p", "miR-30a-5p", "miR-30d-5p", and "miR-10b-5p". The estimated density is displayed on the y-axis, Ct values are displayed on the x-axis. The map also shows different peaks and distribution patterns indicating different features of their expression. (C) Distribution of Cycle threshold (Ct) across four different classes BPH, BCa, control, and PCa where we have compared ‘control with BCa’ and ‘BPH with PCa’. 2D: RefFinder ranking index on dataset three (Rank followed as miR-30a-5p, miR-10b-5p, let-7c-5p, miR-16.

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