Figure 3 | Scientific Reports

Figure 3

From: Identification of organs of origin of macrophages that produce presepsin via neutrophil extracellular trap phagocytosis

Figure 3

Evaluation of P-SEP levels in inhibiting NET formation, M1 MΦ phagocytosis, and protease inhibition. (a) Evaluation of culture medium supernatant P-SEP levels after adding various inhibitors. (b) Western blotting of P-SEP protein levels in M1 MΦs after adding various inhibitors. P-SEP protein levels were quantified using Image J. β-actin band intensity was used to correct P-SEP band intensity. The unprocessed western blot image is shown in Supplementary Fig. S12. All differences denoted by asterisks were subjected to two-tailed Student’s t-tests. Values are presented as mean ± SD (n = 3). **p < 0.01. P-SEP, presepsin; NET, neutrophil extracellular trap; M1 MΦs, M1 macrophage.

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