Figure 1
From: Fabrication of ECM protein coated hollow collagen channels to study peripheral nerve regeneration
Overview of fabrication of 3D collagen gel with hollow channel. (A) 21G Blunt-end needles (green in color) were inserted into a polydimethylsiloxane (PDMS) mold and a 25G blunt end needle (blue in color) was threaded through the pair of 21G needles. A collagen gel was cast around the needles. (B) The 25G needle was removed after polymerization to create hollow channels. Channels were coated in various ECM proteins, where needed. They were incubated and the gel was removed from the PDMS mold into 24 well plates for culture. In all cases, panels with prime designations are representative photographs of their respective schematics.
