Figure 3
From: Immunological assay using a solid-state pore with a low limit of detection
Quantitative analysis of mixed solutions with different mixing ratios of beads with diameters of 300 and 500 nm. (a) Pulse height (Ip) histogram obtained by measuring mixed solutions with different mixing ratios of beads with diameters of 300 and 500 nm. The bead concentration was adjusted to 4 × 109 beads/mL. Measurements at each mixing ratio were made using three solid-state pore modules. The dotted line indicates the threshold (3 nA) that distinguishes 300-nm-diameter beads from 500-nm-diameter beads. N and k denote the total number of pulses obtained in the measurement and the number of pulses attributed to 500-nm-diameter beads, respectively. (b) Relationship between the mixing ratio of 500-nm-diameter beads estimated by the solid-state pore measurement and the actual mixing ratio. Error bars indicate 1× SD. (c) Correlation between the mixing ratio of the beads and pulse frequency. Error bars indicate 1× SD.
