Figure 6
From: Immunological assay using a solid-state pore with a low limit of detection
Flow dynamics of nanoparticles. (a) The three flows that contribute to the flow dynamics of nanoparticles when hydraulic pressure is applied to the cis chamber side. Negatively charged nanoparticles are subjected to electrophoresis and hydraulic pressure in the downward direction from the top. The direction of the electroosmotic flow is from the bottom to the top because cations accumulate at the solid–liquid interface of solid-state pores with negative surface charges. (b) Dependence of pulse frequecy. The error bars indicate ± 3 SD. Sample volume dependence of (c) pulse height (Ip) and (d) pulse width (td) at 0.1 V. Voltage dependence of (e) Ip and (f) td for a sample volume of 15 μL. Voltage dependence of (g) Ip and (h) td for a sample volume of 18 μL. For all pore measurements, the trans chamber volume was 15 μL.
